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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Microglia–Müller Glia Cell Interactions Control Neurotrophic Factor Production during Light-Induced Retinal Degeneration
doi: 10.1523/jneurosci.22-21-09228.2002
Figure Lengend Snippet: Figure 1. Activation of microglia during light-induced retinal degeneration. A–F, Immunohistochemical analysis of normal (A–C) and light-reared (D–F) P35 rat retina using the antibodies iba1 (red in A, C, D, F) and ED1 ( green in B, C, E, F). In light-degenerated retina, iba1 immunoreactivity was observed in the outer retina and double-labeled with ED1 ( yellow) (F, arrowheads). G, Quantitative analysis of the ED1-positive cultured microglial cells from normal (red bar) and light-reared ( green bar) P35 rat retina. Each data point represents the mean SEM of the values obtained from six independent experiments; *p 0.05. H, I, Double-label immunocytochemistry of cultured microglial cells using the antibodies iba1 (red) and ED1 ( green) from normal (H) and light-reared (I) P35 rat retina. GCL, Ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars, 30 m.
Article Snippet: They were then incubated overnight with a microgliaspecific rabbit polyclonal antibody, iba1 (1.0 g/ml) (Graeber et al., 1998; Ito et al., 1998, 2001; Nakajima et al., 1998) and a
Techniques: Activation Assay, Immunohistochemical staining, Labeling, Cell Culture, Double-label Immunocytochemistry
Journal: Pharmaceutical Biology
Article Title: Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
doi: 10.1080/13880209.2017.1390589
Figure Lengend Snippet: Immunostaining of ED-1 positive cell in the liver. (A) Normal; (B) control diabetic; (C) diabetic + TGP 50 mg/kg; (D) diabetic + TGP 100 mg/kg; (E) diabetic + TGP 200 mg/kg. Original magnification 100×.
Article Snippet:
Techniques: Immunostaining
Journal: Investigative ophthalmology & visual science
Article Title: In Vivo Imaging of Fluorescent Probes Linked to Antibodies Against Human and Rat Vascular Endothelial Growth Factor.
doi: 10.1167/iovs.15-18118
Figure Lengend Snippet: FIGURE 5. Choroidal and retinal flatmount staining prepared 7 days following intravitreal injection of bevacizumab-6S-ICC (Cy3-type ICC ¼ indocarbocyanine, red). Staining with CD68 (green, first two rows) showed a colocalization of antibody-conjugates and macrophages, especially within the laser lesions. Staining with Iba1 (green, last two rows) showed also a colocalization with microglial cells. Arrow shows an amoeboid Iba1- positive cell with four small bevacizumab-6S-ICC spots inside (inserted detailed figure).
Article Snippet: Frozen sections of 10 lm were mounted on a glass slide, fixed with 100% methanol and labeled with DAPI (DAPI BioChemica, 40,6-Diamidino-2-phenylindole dihydrochloride; AppliChem GmbH, Darmstadt, Germany),
Techniques: Staining, Injection
Journal: Investigative ophthalmology & visual science
Article Title: In Vivo Imaging of Fluorescent Probes Linked to Antibodies Against Human and Rat Vascular Endothelial Growth Factor.
doi: 10.1167/iovs.15-18118
Figure Lengend Snippet: FIGURE 6. Detection of bevacizumab-6S-ICC (red) in frozen cross sections 7 days following intravitreal injection. In the DAPI stained cross section (A), fluorescent bevacizumab-6S-ICC spots were visible, especially in the area of the laser lesion. Staining for Iba1 shows a moderate colocalization of antibody-conjugates and microglial cells ([B], yellow). A distinct positive double staining with bevacizumab-6S-ICC and CD68 positive macrophages is obvious at the site of CNV (C).
Article Snippet: Frozen sections of 10 lm were mounted on a glass slide, fixed with 100% methanol and labeled with DAPI (DAPI BioChemica, 40,6-Diamidino-2-phenylindole dihydrochloride; AppliChem GmbH, Darmstadt, Germany),
Techniques: Injection, Staining, Double Staining